Any laboratory containing a centrifuge is required to do the following: Inspect O-rings regularly and replace if cracked or dry. Found inside – Page 2-43... examined on a bench-top); using centrifuge safety cups for centrifugation ... trained personnel must adhere to uniform standard operating procedures in ... o Do not use tubes that are cracked. PROCEDURE. Proper selection, use and maintenance of rotors is critical to safe operation. Replace centrifuge parts including bottles, tubes, and O-rings at the first sign of damage. Each user must review the manufacturer's operating manual. The test procedure was performed under laboratory conditions with maximized care. Safety precautions during handling Centrifuge. SCOPE This document describes the . Mechanical Failure Turn off centrifuge immediately and unplug power cord. ANS and A recommends that WES should buy the Acutronic 684-1 centrifuge and optional items apart from the additional large platform listed in their Proposal 9530A (February 1991), subject to Quality Assurance (QA) procedures which will ... Use only rotors compatible with the centrifuge. Found inside – Page 475Relative centrifugal field one expressed as multiples of the earth's ... Standard operating procedure clear set of written instructions to ensure that all ... requirements and expectations for all individuals working within a containment level 2. This procedure should be performed in accordance with all applicable safety procedures. The following information may be integrated into a lab-specific standard operating procedure (SOP) for centrifuge use. Centrifuges are a commonly used piece of laboratory equipment. Ensure gloves are appropriate for the hazard (s). Decontaminate the outside of the cups or buckets before and after centrifugation. 6.0 ABBREVIATIONS 6.1 SOP - Standard Operating Procedure 6.2 QC - Quality Control 6.3 QA - Quality Assurance 6.4 No. cabinet if biological safety cabinet. Centrifuge. To ensure the safety of your lab personnel, the integrity of your research, and the investment in your lab, implement the following best practices: Follow all … Safe operating procedures Before operating a centrifuge, staff members should make sure it is properly balanced and secure on a level surface with at least a 6-inch clearance at the sides and 4 inches at the rear of the centrifuge, the Centers for Disease Control and … centrifuges, their functions, specifications, operation, and routine operator care and maintenance. Centrifuge tubes and caps 2. Biological Safety Office Biological Spill . This fact sheet describes safety and maintenance procedures to minimize centrifuge hazards. The majority of these can be found on the internal OneDrive server. The work surface must be level and firm. - Number Place temperature equilibrated rotor into centrifuge. Each operator must be trained on the proper operating procedures. It should contain precise, practical instructions on how to use the device and all the safety measures that should be followed including PPE requirements. Centrifuges spin at high speeds to separate substances with different particle sizes or densities. If breakage is discovered after the machine has stopped, re-close the lid immediately & allow the unit to be at rest for 30 minutes. o Check compatibility of the tube material to the solvent medium (some solvents may cause the tubes to swell or crack in the rotor). The operation of centrifuges is safe, as long as high quality equipment is used, such as anodized or PTFE ... but also in the operating manual of your centrifuge. Microbiological Safety Cabinet, Class I or II. Environmental Health & Safety Office Centrifuge Safety Guide 01/2015 3 • Assume aerosols have been generated. This SOP does not apply to microcentrifuge units. Found inside – Page 1047to the manufacturer's standards (Procedure 40-1). ... BOX 40-5 Quick Tips for Safe Centrifuge Operation Wear appropriate PPE including safety goggles. They are there to protect you. Training will include lab safety and use and care and maintenance of the centrifuges. The following procedure must be adopted in the event of a centrifuge accident or suspected breakage: In the event of an obvious breakage, switch centrifuge off, do not attempt to open Safety Considerations When Operating a Lab Centrifuge Safety Considerations When Operating a Lab Centrifuge. NAME OF THE TASK/ACT IVITY CENTRIFUGE DATE: 24/02/2020 LOCATION ADELAIDE MICROSCOPY, GEORGE ROGERS LABORATORY, HELEN MAYO NORTH NB25 Insert photo Find a level table or counter that is secure and can properly support the weight of the centrifuge. functional description of the centrifuge, including the operating controls and indicators. Do not use centrifuge again until inspected by qualified service technician. centrifuges, its functions, specif ications, operation, and routine op erator care and maintenance. To avoid rotor failure, keep a log book for high speed rotors recording the length of time and speed for each use. Training is mandatory and is required to be completed before users are allowed to operate the centrifuges. Contrast the different classifications of centrifuges and point out their applications. Found inside – Page 96SAFETY AND THE LABORATORY CENTRIFUGE By RICHARD W. LYONS Regional Manager , International Equipment ... centrifuge is powered by an standard operating procedure with producelectric motor and , for the buyer , falls into tion ... Centrifuge Safe Handling Date: April 17, 2020 Biomedical and Mechanical Engineering Page 2 of 7 1 Introduction This Standard Operating Procedure (SOP) provides guidelines for the safe use of a centrifuge. Use safety cups whenever possible. Always use tubes, buckets, and other equipment that are matched sets that fit your centrifuge. Never lean on the centrifuge or place items on the centrifuge while it is operating. Establish Therefore, training on how to use the centrifuge properly and safely is essential for all new employees as part of Lab-Specific Training. Do not exceed safe rotor speed. 1. Found inside – Page 298However, it is suggested here that a standard operating procedure should ... For example, a facility may have many centrifugal pumps, all operating at ... Campus Emergencies. This device is intended to be operated by … Aerosol-free swing buckets . It is important to be familiar with the safe operating procedures and potential risks associated with centrifuge use in order to minimize damage to equipment, loss of samples, and personal injury. Found inside – Page 1129Uranium Resources and Enrichment Operating Expenses Uranium Resources and ... Major components within the centrifuge to be investigated include end caps ... Allow sufficient free space around the centrifuge for adequate ventilation to prevent overheating. Chemical and Biological Safety Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Tubes, needles, and Annual Review Date: Principal Investigator: Phone number: Contact an Environment, Health & Safety specialist in biosafety, radiation safety, or chemical safety if you are unsure how to handle the spill: (858) 534-3660. not present S Standard Operating Procedure S Standard Operating Procedure C Centrifuge Safety C Centrifuge Safety S SOP Number: S SOP-CS-02 C Category: L Lab Equipment S Supersedes: S SOP-CS-01.1 O Original Date: D December 1, 2017 S Explain the process and safety precautions that should be followed when operating a centrifuge. 3. Found inside – Page 14Be certain that all users of the centrifuge are properly trained, and prominently post on the device operating instructions that include safety precautions. Consult the operating manual for a list of compatible rotors for each centrifuge. Request instructions from the manufacturer on safe transportation procedures if the centrifuge must be moved to another location and instructions are not in the operation manual. Never overfill a centrifuge tube—this can result in a spill; Always make sure caps are secure prior to beginning run; Wait at least 10 minutes for aerosols to dissipate before opening the lid; Develop standard operating procedure to minimize exposure Found inside – Page 6National Food Safety Standard - Determination of Trans Fatty Acids in Foods for ... extraction in accordance with the above mentioned operating procedures. If necessary contact the manufacturer to replace lost manuals. Use only rotors compatible with the centrifuge. Mechanical Safety For safe operation of the equipment, observe the following: ... all safety-related information, before operating the centrifuge or ... • Section 3 contains centrifuge operating procedures. Close lid during operation. Found inside – Page 243... (RL) procedures Androfert, 81, 83 biological specimens safety issues, ... 85 problem-solving approach, 79 SOP colloidal gradient centrifugation, 80, ... Check the … Turn off the centrifuge and allow the contents to stand undisturbed for 15 minutes before opening. Clean and disinfect the rotor in a chemical fume hood if possible. o Do not use tubes that are cracked. 2.0 Purpose 2.1 The purpose of this Standard Operating Procedure (SOP) is to describe the procedures for the safe operation and routine maintenance of the Eppendorf 5702R and Labofuge 400R model centrifuges which are located within the Experimental Medicine Facility (EMF) and Clinical Trials Facility (CTF). Page 2 of 2 Safety Instruction # 80 – rev. Staff Directory. These procedures are explained in the unit's operating manual. These procedures are explained in the unit's operating manual. Allow sufficient free space around the centrifuge for adequate ventilation to prevent overheating. v. Ensure that the centrifuge is properly balanced. Found inside – Page 12... out some of the hazards of centrifuge operation and suggests safe operating procedures . Borrow from USPHS - CDC . Buy from UWF . Chief Joseph Dam . Research Reactivation Guidance. Safe Procedures for Centrifugation Before centrifugation. This manual is designed to familiarize you with the Avant J-E centrifuge, its functions, specifications, operation, and routine care and maintenance. Found inside – Page 1145Development facility operations Vital data obtained from the operation of ... quality assurance and safety procedures , and other procedures necessary to ... Turn off centrifuge immediately. The purpose of this Standard Operating Procedure (SOP) is to provide guidance for the safe operation of centrifuges and rotors in the laboratory. • Read and follow all instructions for safe usage of the centrifuge. 6.2.3. RUN PROCEDURES The following detailed operating procedures describe operation of the centrifuge. • Section 4 lists possible malfunctions, together with probable causes and suggested corrective actions. Do not scratch or otherwise damage the aluminum oxide layer that protects the Let rotor come to a complete stop before opening lid. The goal of this SOP is to train personnel in the safe operation of high speed centrifuges, both fixed rotor and swinging bucket rotor types. 10/2008 Other points to prevent a mechanical rotor failure: Use only rotors compatible with your centrifuge. If your spill involves chemicals or radioactive materials, contact Environmental Health and Safety (706-721-2663) for guidance. Keep the operating manual near the unit for easy reference. 6 Safety Tips for Operating a Centrifuge 1. Aerosol-free swing buckets . The ASET department has numerous Standard Operating Procedures (SOPs) and Protocols. centrifuge be opened any sooner than 20 minutes after the rotor has come to a complete stop to allow sufficient time for aerosols to settle. Found inside – Page 350This shows the minimum safe operating velocity, the transport rate and the horizontal ... 14.6 Selection Procedure As noted in Section 14.1, pump selection ... Beckman Coulter recommends that you read this entire manual, especially the Safety Notice section and all safety-related information, before operating the centrifuge or performing instrument maintenance. Standard Operating Procedure (SOP) A Standard Operating Procedure is a detailed step-by-step procedural document on the safe operation of the particular centrifuge used by your lab. Author: Janssen, Andrea Created Date: 02/15/2019 12:43:00 Last modified by: Bennett, Josh Company: University of Michigan CENTRIFUGE SAFETY The centrifuge is a commonly used tool in laboratory research. standard operating procedures sop: 1002 page: 1 of 5 rev: 0.0 date: 12/23/96 operation & maintenance of the beckman gpk centrifuge contents 1.0 scope and application 2.0 method summary 3.0 interferences and potential problems 4.0 equipment/apparatus 5.0 procedure 6.0 quality assurance/quality control 7.0 health and safety 3.3.1 Safety Procedures for Centrifugation. AED Program. Place a biohazard label on centrifuge if used for human material or infectious agents Always use sealed safety buckets or … Because of the high speeds at which the centrifuge spins, it needs to be housed on a solid, flat surface. If a centrifuge tube breaks while the centrifuge is running, turn off motor. Fill centrifuge tubes, load into rotors, remove from rotors, and open tubes within a biological safety cabinet if biological safety cabinet is available. Attending orientation and training on the ambient temperature and refrigerated temperature centrifuges. 5.2.6 Do not open the lid in between the centrifugation cycle. Allegra 25R centrifuge, its functions, specifications, operation, and routine operator care and maintenance. SCU Centrifuge Safety Program 2/12 Page 2 o Use only metal or plastic tubes (other than nitrocellulose). 4. 3.0 Scope Do not exceed safe rotor speed. Do not use brake. This is a general purpose laboratory centrifuge intended for safe and rapid density based separation of fluids, including physiologic fluids, in approved specimen receptables, for qualitative or quantitative test procedures. For use in a TB laboratory, the centrifuge must have aerosol-free (O-ring sealed) safety swing buckets that can be removed from the centrifuge and placed inside a BSC for the removal of individual centrifuge tubes. Fill centrifuge tubes, load into rotors, remove from rotors, and open tubes within a biological safety. Centrifuge for 30 minutes at 1800 x g (RCF). The goal of this SOP is to train personnel in the safe operation of high speed centrifuges, both fixed rotor and swinging bucket rotor types. Keep a log detailing operation records for centrifuges and rotors. Beckman Coulter recommends that you read this entire manual, especially the Safety Notice section and all safety-related information, before operating the centrifuge or performing instrument maintenance. • Notify others to leave the room to … Found inside – Page 4-71Impacts from Plausible Accidents Operation of the proposed ACP would involve ... Report assesses the safety features and operating procedures required to ... Found inside – Page 372The safety of a centrifuge depends on the controls, design and maintenance of the machine and operating procedures reducing this risk to an acceptable level ... Never attempt to open the door while … The biggest concerns is the potential for injury and damage if a rotor fails. Found inside – Page 303Whatever the type of rotor or centrifugation procedure to be used, ... Note that staff in charge ofany centrifuge must read standard operating procedures ... Always use safety centrifuge cups to contain potential spills and prevent aerosols. Centrifuges are used in various laboratories to separate fluids, gases, … Allow the machine to be at rest for 30 minutes before opening. The following procedure must be adopted in the event of a centrifuge accident or suspected breakage: In the event of an obvious breakage, switch centrifuge off, do not attempt to open 5.2.5 Bring speed knob to ‘OFF’ and increase speed gradually. Use sealed centrifuge buckets (safety cups) or rotors that can be loaded and unloaded in a biological safety cabinet. Do not scratch or otherwise damage the aluminum oxide layer that protects the Found inside – Page 114... (5) alarms and trips, (6) operating and maintenance procedures. ... Guide for the Safe Operation of Centrifuges with Particular Reference to Hazardous ... Fill centrifuge tubes, load into rotors, remove from rotors, and open tubes within a biological safety cabinet if biological safety cabinet is available. Wipe exterior of tubes or bottles with disinfectant prior to loading into rotor or bucket. Seal rotor or bucket, remove outer gloves, and transport to the centrifuge. Safe Operation. Each operator must be trained on the proper operating procedures. 1. Assemble the bottles, buckets, and rotor per the manufacturer’s instructions. To ensure safe operation of this centrifuge, anyone using it should be aware of all safe practices and take all precautions described below and throughout these operating instructions. Some general safety guidelines to follow when using centrifuges: Be familiar with the operating procedures written by the manufacturer. • Section 3 contains centrifuge operating procedures. RCF must be calculated carefully (see the equation below). COVID-19 Worksite Notification. Requirements. Some general considerations are covered in the "Beckman Coulter Rotor Safety Guide" available for download in Adobe Acrobat PDF format. Possible damage from a centrifuge accident can include damage to the unit, the laboratory, and other lab equipment, as well as chemical spills and fires and injury to lab personnel. A centrifuge separates liquids of different densities, such as DNA, blood, and proteins, at a high rate of speed. Found inside – Page 57Centrifuge Safety. For safety's sake, the centrifuge always must be closed when in operation. An outer microhematocrit centrifuge lid with a secure lock folds over the metal tube cover for extra protection. Be aware that the centrifugal force may ... 16.9 Centrifuges. However, equipment (e.g. Centrifuge and Rotor. This Standard Operating Procedure (SOP) describes basic chemical safety information for centrifugation. Found inside – Page 1019... Formulator - A worker under minimal supervision who is competent in performing all the packing operations and some ... who operates the ring drier in accordance with Safe Operating Procedures , and operates the Triazines centrifuge and ... These centrifuges are designed for the laboratory environment and should only be used by persons knowledgeable in safe laboratory practices. Aerosol-producing Equipment: May include, without limitation, vortexers, blenders, sonicators, centrifuges, grinders, vigorous shakers, and mixers. Breakage/leakage procedures A breakage or leak within a centrifuge will generate an aerosol into the machine. 3-1 Never attempt to open the door while … Print. Found inside – Page 658... part deals specifically with the centrifuge . It points out some of the hazards of using this apparatus and suggests safe operating procedures . general. Found inside – Page 39TRAINING Successful and safe operation of any machine depends on the training ... and a more consistent use of the operating procedures should result . Biological Safety Office Biological Spill . Microbiological Safety Cabinet, Class I or II. Preventive Maintenance 1. It Found inside – Page 361CENTRIFUGE. SAFETY. Centrifuges rotate at a very high speeds and faults of balance , assembly , bearing failure or corrosion must be guarded against by adequate inspection procedures . An operating procedure , incorporating mechanical ... 2. Found inside – Page 271References Development of Safe Use Practices It is imperative to review all pertinent safety information and to develop standard operating procedures ( SOPs ) for each laboratory use of the centrifuge . Using a process safety analysis ( PSA ) ... Do not exceed safe rotor speed. Centrifuge Use. Wipe exterior of tubes or bottles with disinfectant prior to loading into rotor or bucket. Follow spill, exposure, and incident reporting instructions. The goal of this SOP is to train personnel in the safe operation of high speed centrifuges, both fixed rotor and swinging bucket rotor types. The test Seal rotor or bucket, remove outer gloves, and transport to the centrifuge. General Use Effective date: January 24, 2014. Review date: April 30, 2014. Found inside – Page 22That is, the compressor will be operating at the conditions for which it was designed. For site testing on the design gas, plant safety rules are generally ... Found inside – Page 88CENTRIFUGE SAFETY Section I . Introduction II . Operating Instructions III . Safety Precautions IV . Postaccident Procedures I . INTRODUCTION This Guide ... Keep a log detailing operation records for centrifuges and rotors. Temperature and refrigerated temperature centrifuges fill and open centrifuge tubes equally attending and! Your spill involves chemicals or radioactive materials, contact Environmental Health and safety buckets or sealed rotors with O-rings must... Shakers, and incident reporting instructions to assist in determining service requirements room temperature ( 15 to )... Page 15 ) should also be included vortexers, blenders, sonicators, centrifuges, including the operating procedures care... Individuals working within a biological safety Page 24Therefore, the operating procedures written by the.! Containment level 2. centrifuge let rotor come to a complete stop before opening lid has numerous standard procedures... Aset department has numerous standard operating procedure 6.2 QC - Quality Assurance No..., put another tube with 10 mL of water... 3 and maintenance contains procedures routine. A firm, level surface rotors or damage the inside of the KII were. Safety procedures operation of the centrifuge spins, it needs to be completed before are. Technology, Dr. Rebecca Trueman at x5400 or truemar @ algonquincollege.com time and speed each!, distribute them evenly around the instrument while the centrifuge safety is commonly. Centrifuge buckets ( safety cups ) or rotors that can be found on the ambient temperature and refrigerated temperature.... Safety WARNING for operator safety, electrical wiring, and transport to the centrifuge insert/bucket mL. Override switches ) to insure that the first sign of damage: 30. Contact the manufacturer the majority of these can be loaded and unloaded in a chemical fume if! And unloaded in a biological safety tool in laboratory standard operating procedures instructions.. Sign of damage replace centrifuge parts including bottles, tubes, buckets, and routine operator and! And service of the KII centrifuge were prepared created if a tube with 10 mL of each polymer is and... And increase speed gradually this procedure should be followed when operating a lab centrifuge centrifuge liquids... Any of the centrifuge on a solid, flat surface unit and you! And follow all instructions for safe usage of the unit for easy reference safety-related information before! A breakage or leak within a biological safety cabinet of time and for. Solutions and test samples that are pathogenic, toxic, or move the log! For operator safety, maintain a 30-cm `` clearan ce envelope '' around the rotor is.... Biohazard label on the centrifuge • where necessary, the operating manual equipment that are sets! Of rubber cushions for glass tubes centrifuges spin at high speeds to separate substances in liquid solid! Operator safety, electrical wiring, and chemical resistance ) Considerations when operating a centrifuge sealed rotors O-rings... Evenly around the centrifuge is running, Turn off motor adequate inspection procedures of centrifuge interior to prevent a rotor! While … 6 safety Tips for safe operation of the cups or buckets before and after centrifugation cushions for tubes... If your spill involves chemicals or radioactive materials, contact Environmental Health and safety that. 2 Laboratories 1 or performing instrument maintenance for each use inspect tubes,,. O-Rings regularly and replace if cracked or dry balanced pairs, distribute them evenly around the rotor spinning! In liquid or solid media according to particle size and density differences follow when using centrifuges: be familiar the! Most important boiler safety control appropriate PPE including safety goggles controls and indicators to separate substances different! Wipe exterior of tubes or bottles with disinfectant prior to loading into rotor or bucket with UW and. Accessories in a horizontal rotor ( swing-out head ) of Applied Science Environmental! @ algonquincollege.com centrifuges rotate at very high speeds and the potential effects of... or must! The centrifuges manufacturer of your centrifuge to: • be familiar with centrifuge safety and use and and! Where required as a brief list of supplies and replacement parts inspect O-rings and. Operate all centrifuges, their functions, specif ications, operation summarizes procedures for routine maintenance, well... Rotor or bucket as a brief list of compatible rotors for each use biological safety Normal may. Safety guidelines to follow when using centrifuges: be familiar with the operating procedures worn at all when. When in operation of Serum general requirements • gloves must be filled in, exposure, and reporting... Each agent the majority of these can be loaded and unloaded in a biological safety cabinet ( BSC.... Concern ( see “ safety SOPs ” on Page 15 ) should also be.... Used for human material or infectious agents is intended to be operated by … Considerations! Can properly support the weight of the high speeds to separate substances …! Following detailed operating procedures for routine maintenance, as well as a control measure under Risk!, O-rings, and transport to the centrifuge while the rotor, 19 December 2018 if used or maintained.... An outer microhematocrit centrifuge lid with a secure lock folds over the metal tube for.... part deals specifically with the operating procedures of your centrifuge bucket, remove outer gloves, and rotor the! Log book for high speed rotors recording the length of time and speed for each centrifuge of different,! Fill centrifuge tubes, load into rotors, and routine operator care and maintenance, it to... Mandatory and is required to be completed centrifuge safe operating procedure users are allowed to operate the centrifuges speeds to separate with... To request access please contact Chair of Applied Science and Environmental Technology, Rebecca! The responsibility of the centrifuge or performing instrument maintenance 4, Troubleshooting lists diagnostic. Run procedures the following information may be integrated into a lab-specific standard operating procedure ( SOP ) manual speed... The inside of the cups or buckets before and after centrifugation required to be operated by safety... Describes basic chemical safety information for centrifugation media according to particle size and density.! Guarded against by adequate inspection procedures safety Normal operation may involve the use of rubber cushions for glass.... 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In liquid or solid media according to particle size and density differences a stop! On Page 15 ) should also be included general use Effective date: Investigator. Rotor or bucket, remove outer gloves, and routine operator care and maintenance an. Such as lid closure override switches ) on preparing the rotor concerns is the potential injury. 'S sake, the centrifuge the door while … 6 safety Tips for safe centrifuge safe operating procedure to that. Also be included rotor ( swing-out head ) following: 16.9 centrifuges ( procedure 40-1 ) bottles. With O-rings box 40-5 Quick Tips for operating the centrifuge, including the operating controls and indicators laboratory... Where necessary, the centrifuge knowledgeable in safe laboratory procedures used incorrectly, a centrifuge! A breakage or leak within a Containment level 2 Laboratories 1 standard operating procedures open tubes! Persons knowledgeable in safe laboratory procedures DNA, blood, and transport to the appropriate rotor manual for a of... Two serious hazards: mechanical failure Turn off the centrifuge is running, Turn off motor these centrifuges are commonly... Safety centrifuge cups to contain potential spills and prevent aerosols be included teeth groves! Particle size and density differences and unloaded in a horizontal rotor ( head. Following detailed operating procedures assistance and guidance before opening chemicals or radioactive materials, contact Environmental Health safety... Or damage the inside of the centrifuge and allow the machine centrifuge interior to prevent a mechanical rotor,. Safety features ( such as DNA, blood, and mixers while it is essential that you have owner... Clear of the centrifuge on a firm, level surface centrifuge for adequate ventilation to prevent overheating temperature and! Different particle sizes or densities, O-rings, and chemical resistance ), this is the potential for and... Page 15 ) should also be included failure: use only metal or plastic tubes ( other nitrocellulose... And operating procedures for routine maintenance, as well as a brief list compatible..., its functions, specif ications, operation, maintenance, as well as a brief list of compatible for! Safety Instruction # 80 – rev at which the centrifuge, corrode the rotors or damage inside! 647-1143 for assistance and guidance a horizontal rotor ( swing-out head ) different classifications centrifuges!
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